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Test Code HODGE Carbapenemase Detection, Modified Hodge Test

Biohazard Infectious
Specimen

Reporting Name

Carbapenamase-Modified Hodge Test

Useful For

Determining carbapenem resistance

Method Name

Modified Hodge Test

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies

Specimen Required

Container/Tube: Slant

Specimen Volume: Isolate

Collection Instructions:

1. Submit Enterobacteriaceae species in pure culture, actively growing.

2. Place specimen in a large infectious container (Supply T146) and label as an etiologic agent/infectious substance.

Additional Information:

1. Specimen source and organism identification are required.

2. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

Forms: If not ordering electronically, submit a Microbiology Request Form (Supply T244) with the specimen.

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Sunday

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87185

LOINC Code Information

Result ID Reporting Name LOINC Code
HODGE Carbapenamase-Modified Hodge Test In Process

Clinical Information

Resistance to antibiotic therapy via production of the enzyme carbapenemase by Klebsiella pneumoniae and other members of Enterobacteriaceae is becoming more common. This resistance is not always detected by conventional antimicrobial susceptibility testing, which may result in inappropriate antimicrobial therapy for the patient.

 

In Enterobacteriaceae, the gene blaKPC, which encodes KPC (Klebsiella pneumoniae carbapenemase) production, can be detected by real-time PCR. However, molecular methods have not been established for other carbapenemases.

 

The modified Hodge test, a phenotypic method, is recommended by the Clinical and Laboratory Standards Institute (CLSI) as the method to detect carbapenemases.

Interpretation

A positive result indicates the production of carbapenemase.

 

A negative result indicates the lack of production of carbapenemase.

Analytic Time

2 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Agar plate

Additional Tests

Test ID Reporting Name Available Separately Always Performed
HODGB Carbapenamase-Modified Hodge Test No, (Bill Only) Yes