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Test Code MYCPN Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum

Reporting Name

M. pneumoniae Ab, IgG and IgM, S

Useful For

An aid in the diagnosis of disease associated with Mycoplasma pneumoniae

Profile Information

Test ID Reporting Name Available Separately Always Performed
MYCG M. pneumoniae Ab, IgG, S No Yes
MYCM M. pneumoniae Ab, IgM, S No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MMYCO M. pneumoniae Ab, IgM, S by IFA No No

Testing Algorithm

If Mycoplasma pneumoniae antibodies, IgM is positive or equivocal, then Mycoplasma pneumoniae antibodies, IgM by indirect immunofluorescence assay (IFA) will be performed at an additional charge.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required

Collection Container/Tube: 

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values


≤0.90 (negative)

0.91-1.09 (equivocal)

≥1.10 (positive)



≤0.90 (negative)

0.91-1.09 (equivocal)

≥1.10 (positive)


IgM by IFA

Negative (reported as positive or negative)

Day(s) and Time(s) Performed

Monday through Friday; 9 a.m.

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86738 x 2-Mycoplasma pneumoniae by EIA

86738-Mycoplasma pneumoniae by indirect IFA (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MYCPN M. pneumoniae Ab, IgG and IgM, S In Process


Result ID Test Result Name Result LOINC Value
MYCG M. pneumoniae Ab, IgG, S 5255-5
MYCM M. pneumoniae Ab, IgM, S 5256-3

Clinical Information

Mycoplasma pneumoniae is an important respiratory tract pathogen.


Several syndromes have been associated with the infection including pharyngitis, tracheobronchitis, pneumonia, and inflammation of the tympanic membrane presenting as bullous myringitis.  


Mycoplasma pneumoniae accounts for approximately 20% of all cases of pneumonia. Classically, it causes a disease that has been described as primary atypical pneumonia. The disease is of insidious onset with fever, headache, and malaise for 2 to 4 days before the onset of respiratory symptoms. Most cases do not require hospitalization. Symptomatic infections attributable to this organism most commonly occur in children and young adults (ages 2-19 years).(1)


Positive IgM results are consistent with acute infection, although false positives do occur (see Cautions).


A single positive IgG result only indicates previous immunologic exposure.


Negative results do not rule-out the presence of Mycoplasma pneumoniae-associated disease. The specimen may have been drawn before the appearance of detectable antibodies. If testing is performed too early following primary infection, IgG and/or IgM may not be detectable. If a Mycoplasma infection is clinically indicated, a second specimen should be submitted in 14 to 21 days.

Analytic Time

Same day/1 day

Specimen Retention Time

14 days

Reject Due To


Mild reject; Gross reject


Mild reject; Gross reject





Method Name

MYCG, MYCM: Enzyme Immunoassay (EIA)

MMYCO: Indirect Immunofluorescence Assay (IFA)