Test Code 1013 Tzanck Smear
Methodology
Screening and Interpretation by Pathologist
Performing Laboratory
Alomere Health Hospital Laboratory
Specimen Requirements
Specimen Type: Scrape/brushing from lesion
Preferred: CytoLyt® Fixative
1. Gently scrape lesion with cyto brush.
2. Immediately swish brush into CytoLyt® fixative.
2. Label CytoLyt® container with patient identification and specimen source.
Note: 1. Specify specimen source on requisition and label all containers.
2. Please complete a “Cytology Examination Request Form” in “Forms” in “Special Instructions” and forward it with the specimen.
Acceptable: Direct Smears
1. Label slides with patient identification.
2. Gently scrape lesion with scraper or a non-absorbent, cotton-tip applicator premoistened with saline.
3. Immediately smear specimen onto glass slide with a single smooth motion when using a scraper and a rolling motion when using the cotton-tip applicator.
4. Immediately place slides into 95% alcohol to avoid air drying and distortion of cells.
5. Specify specimen source on requisition and label all slides and containers.
6. Complete a “Cytology Examination Request Form” in “Forms” in “Special Instructions” and forward it with the specimen.
Specimen Transport Temperature
Ambient
Reference Values
The presence of multi-nucleated giant cells with cytopathic effect is indicative of herpes.
Day(s) Test Set Up
Monday through Friday
Test Classification and CPT Coding
88160 - Cytopathology smears, other source (if appropriate)
88112 - Nongyn, Thinprep® (if appropriate)