Specimen Requirements

Specimen Type: Breast fluid, Nipple discharge

Preferred: CytoLyt® Fixative

1. Express up to 20 mL cellular material into CytoLyt® fixative. Slides will be prepared at Alomere Health Pathology.

2. Label CytoLyt® container with patient identification and specimen source.

3. Specify specimen source on requisition and label all containers.

4. Complete a “Cytology Examination Request Form” in “Forms” in “Special Instructions” and forward it with the specimen.

Direct Smears

1. Label polysine slides with patient identification.

2. Carefully apply a small drop of material onto 1 end of a slide. A second slide is placed on top of first slide to obtain an even spread of material, and then separate the slides. Preparation of smear should be performed gently in order to prevent crushing of cells. Prepare 2 slides.

3. Immediately place slides in 95% alcohol fixative.

4. Place up to 20 mL of remaining fluid in CytoLyt® fixative.

5. Specify specimen source on requisition and label all slides and containers.

6. Complete a “Cytology Examination Request Form” in “Forms” in “Special Instructions” and forward it with the specimen.