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Test Code AMOBS Amobarbital, Serum

Reporting Name

Amobarbital, S

Useful For

Monitoring amobarbital therapy

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions:

1. Draw blood immediately before next scheduled dose.

2. Centrifuge and aliquot serum into a plastic vial within 2 hours of collection.


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Reference Values

Therapeutic concentration: 1.0-5.0 mcg/mL

Toxic concentration: >10.0 mcg/mL

Day(s) Performed

Thursday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AMOBS Amobarbital, S 3338-1

 

Result ID Test Result Name Result LOINC Value
8325 Amobarbital, S 3338-1

Clinical Information

Amobarbital is an intermediate-acting barbiturate with hypnotic properties used in short-term treatment of insomnia and to reduce anxiety and provide sedation preoperatively.(1,2)

 

Amobarbital is administered by intravenous infusion or intramuscular injection. The duration of its hypnotic effect is about 6 to 8 hours. The drug distributes throughout the body, with a volume of distribution of 0.9 to 1.4 L/kg, and about 59% of a dose is bound to plasma proteins. Metabolism takes place in the liver primarily via hepatic microsomal enzymes. Its half-life is about 15 to 40 hours (mean: 25 hours). Excretion occurs mainly in the urine.(2,3)

Interpretation

Amobarbital concentrations above 10 mcg/mL have been associated with toxicity.

Report Available

3 to 9 days

Specimen Retention Time

2 weeks

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Gas Chromatography Mass Spectrometry (GC-MS)

Forms

If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.