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HelpTest Code ETGX Ethyl Glucuronide Confirmation, Chain of Custody, Random, Urine
Specimen Required
Supplies: Chain of Custody Kit (T282)
Container/Tube: Chain-of-custody kit containing the specimen containers, seals, and documentation is required.
Specimen Volume: 5 mL
Collection Instructions: Collect specimen in the provided container, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.
Additional Information: Submitting less than 5 mL will compromise our ability to perform all necessary testing.
Forms
1. Chain of Custody Request is included in the Chain-of-Custody Kit (T282).
2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.
Useful For
Monitoring abstinence in clinical and justice system settings using ethyl glucuronide and ethyl sulfate as direct biomarkers or metabolites of ethanol
This chain-of-custody test is intended to be used in a setting where the test results can be used definitively to make a diagnosis.
Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was always under the control of personnel involved with testing the specimen; this control implies that the opportunity for specimen tampering would be limited.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| COCH | Chain of Custody Processing | No | Yes |
| ADLTX | Adulterants Survey, CoC, U | Yes | Yes |
Testing Algorithm
Adulterants testing will be performed on all chain-of-custody urine samples as per regulatory requirements.
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Ethyl Glucuronide Conf, CoC, USpecimen Type
UrineSpecimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 72 hours |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Clinical Information
Ethyl glucuronide and ethyl sulfate are minor metabolites of ethanol that are detectable in body fluids following alcohol consumption and, less commonly, following extraneous exposure. Ethyl glucuronide (EtG) and ethyl sulfate (EtS) are direct biomarkers or metabolites of ethanol. EtG and EtS can be detected up to 5 days in urine using a cutoff of 500 ng/mL.(1)
Chain of custody is a record of the disposition of a specimen to document each individual who collected, handled, and performed the analysis. When a specimen is submitted in this manner, analysis will be performed in such a way that it will withstand regular court scrutiny.
Reference Values
Negative
Cutoff concentrations: 500 ng/mL
Interpretation
A positive interpretation will be given if either the ethyl glucuronide result is greater than or equal to 250 ng/mL or the ethyl sulfate is greater than or equal to 100 ng/mL.
A "high" positive (ie, >1000 ng/mL) may indicate:
-Heavy drinking on the same day or previously (ie, previous day or 2)
-Light drinking the same day
A "low" positive (ie, 500-1000 ng/mL) may indicate:
-Previous heavy drinking (ie, previous 1-3 days)
-Recent light drinking (ie, past 24 hours)
-Recent intense "extraneous" exposure (ie, within 24 hours or less)
A "very low" positive (ie, 100-500 ng/mL) may indicate:
-Previous heavy drinking (ie, 1-3 days)
-Previous light drinking (ie, 12-36 hours)
-Recent "extraneous" exposure
Day(s) Performed
Monday, Wednesday, Friday
Report Available
3 to 5 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80307
80321
G0480 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ETGX | Ethyl Glucuronide Conf, CoC, U | 93705-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36853 | ETG Immunoassay Screen | 93706-0 |
| 63418 | Ethyl Glucuronide | 58378-1 |
| 36850 | Ethyl Sulfate | 58425-0 |
| 36852 | Chain of Custody | 77202-0 |
| 36851 | Ethyl Gluc/Sulfate Interpretation | 59462-2 |