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Test Code LY27B HLA-B27, Blood

Reporting Name

HLA-B27, B

Useful For

Assisting in the diagnostic process of ankylosing spondylitis, juvenile rheumatoid arthritis, and reactive arthritis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Ordering Guidance


This test is best used alone if a particular disease, such as ankylosing spondylitis, is under consideration.

 

Orders received for both this test and 1LRR / Human Leukocyte Antigens (HLA) Class I Typing Low Resolution, Recipient, Blood or 1DIS / Human Leukocyte Antigens (HLA) A-B-C Disease Association Typing Low Resolution, Blood (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap.



Shipping Instructions


Specimen must arrive within 96 hours of collection.



Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 4 days

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Saturday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86812

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LY27B HLA-B27, B 26028-1

 

Result ID Test Result Name Result LOINC Value
LY27 HLA-B27 Result 26028-1
B27C Interpretation 96625-9

Clinical Information

This major histocompatibility coded class I antigen is associated with ankylosing spondylitis, juvenile rheumatoid arthritis, and reactive arthritis. The mechanism of the association is not understood but probably is that of linkage disequilibrium.

 

There is an increased prevalence of human leukocyte antigen (HLA)-B27 in certain rheumatic diseases, particularly ankylosing spondylitis.

 

Studies have demonstrated that the HLA-B*27:06 allele, which is present in a small percentage of individuals of Asian ethnicity, may not be associated with ankylosing spondylitis.

Interpretation

Approximately 8% of the normal population carries the human leukocyte antigen (HLA)-B27.

 

HLA-B27 is present in approximately 89% of patients with ankylosing spondylitis, 79% of patients with reactive arthritis, and 42% of patients with juvenile rheumatoid arthritis. However, lacking other data, it is not diagnostic for these disorders.

Report Available

2 to 6 days

Specimen Retention Time

7 days

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Method Name

Flow Cytometry

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.