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HelpTest Code MPU Monoclonal Protein Studies, 24 Hour, Urine
Shipping Instructions
Refrigerate specimen during collection and send refrigerated.
Necessary Information
24-Hour volume (in milliliters) is required.
Specimen Required
Supplies: Urine Container, 60 mL (T313)
Submission Container/Tube: Plastic, 60-mL urine bottle
Specimen Volume: 50 mL
Collection Instructions:
1. Collect urine for 24 hours.
2. Aliquot between 30 mL and 50 mL urine into plastic, 60-mL urine bottle.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Useful For
Monitoring patients with monoclonal gammopathies
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MPTU | M-protein Mass-Fix, 24 HR, U | No | Yes |
| PEU | Protein Electrophoresis, 24 Hr, U | No | Yes |
| PTU3 | Protein, Total, 24 HR, U | Yes, (Order PTU) | Yes |
Testing Algorithm
The following algorithms are available:
Special Instructions
Method Name
PTU: Turbidimetry
PEU: Agarose Gel Electrophoresis
MPTU: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
Reporting Name
M-protein Studies, 24 HR, USpecimen Type
UrineSpecimen Minimum Volume
30 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 14 days | |
| Frozen | 5 days | ||
| Ambient | 24 hours |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Clinical Information
Urine proteins can be grouped into 5 fractions by protein electrophoresis:
-Albumin
-Alpha-1
-Alpha-2
-Beta-globulin
-Gamma-globulin
One or more quantifiable monoclonal proteins may be present and reported as M spike.
The urine total protein concentration, the electrophoretic pattern, and the presence of a monoclonal immunoglobulin light chain may be characteristic of monoclonal gammopathies such as multiple myeloma, primary systemic amyloidosis, and light chain deposition disease.
The following algorithms are available:
Reference Values
PROTEIN, TOTAL:
<229 mg/24 hours
Reference values have not been established for patients <18 years of age.
ELECTROPHORESIS, PROTEIN:
The following fractions, if present, will be reported as mg/24 hours:
Albumin
Alpha-1 globulin
Alpha-2 globulin
Betaglobulin
Gamma globulin
MASS-FIX M-PROTEIN ISOTYPE:
M-protein Isotype MS:
No monoclonal protein detected
Flag M-protein Isotype MS:
Negative
Interpretation
The presence of a monoclonal immunoglobulin light chain in the urine is seen in multiple myeloma, macroglobulinemia, primary systemic amyloidosis and light-chain deposition disease, monoclonal gammopathy of undetermined significance, and idiopathic Bence Jones proteinuria. The presence of a monoclonal light chain can produce renal insufficiency, may be deposited as amyloid fibrils, may damage the proximal tubes producing Fanconi syndrome, or light chains may deposit in the glomerulus and cause light-chain deposition disease.
Heavy chain fragments as well as light chains may be seen in the urine of patients with multiple myeloma or amyloidosis.
Day(s) Performed
Monday through Friday
Report Available
4 to 6 daysSpecimen Retention Time
See Individual Test IDsPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84156
84166
0077U
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MPU | M-protein Studies, 24 HR, U | 101910-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 607970 | Albumin | 6941-9 |
| 617117 | M-protein Isotype MS, 24 HR, U | In Process |
| TP6 | Total Protein, 24 HR, U | 2889-4 |
| TM24 | Collection Duration | 13362-9 |
| 617118 | Flag M-protein Isotype MS, 24 HR, U | No LOINC Needed |
| 607971 | Alpha-1 globulin | 6794-2 |
| 607972 | Alpha-2 globulin | 6795-9 |
| VL92 | Urine Volume | 3167-4 |
| 607973 | Beta globulin | 94714-3 |
| 607974 | Gamma globulin | 94715-0 |
| 2833 | A/G Ratio | 44294-7 |
| 21446 | M spike | 42482-0 |
| 22307 | M spike | 42482-0 |
| 21447 | Impression | 32210-7 |
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.