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Test Code SGSU Synthetic Glucocorticoid Screen, Urine

Reporting Name

Synthetic Glucocorticoid Screen, U

Useful For

Confirming the presence of the listed synthetic glucocorticoids (see Interpretation)


Confirming the cause of secondary adrenal insufficiency

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Specimen Required

Container/Tube: Plastic, 10-mL urine tube (T068)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.

Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Frozen 14 days

Reference Values


Cutoff concentrations

Beclomethasone dipropionate: 0.10 mcg/dL

Betamethasone: 0.10 mcg/dL

Budesonide: 0.20 mcg/dL

Dexamethasone: 0.10 mcg/dL

Fludrocortisone: 0.10 mcg/dL

Flunisolide: 0.10 mcg/dL

Fluorometholone: 0.10 mcg/dL

Megestrol acetate: 0.10 mcg/dL

Methylprednisolone: 0.10 mcg/dL

Prednisolone: 0.10 mcg/dL

Prednisone: 0.10 mcg/dL

Triamcinolone 0.30 mcg/dL

Triamcinolone acetonide: 0.10 mcg/dL


Values for normal patients not taking these synthetic glucocorticoids should be less than the cutoff concentration (detection limit).

Day(s) and Time(s) Performed

Tuesday, Thursday; 9:00 a.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
SGSU Synthetic Glucocorticoid Screen, U 46959-3


Result ID Test Result Name Result LOINC Value
23561 Beclomethasone Dipropionate 46945-2
23562 Betamethasone 46946-0
23563 Budesonide 46947-8
23564 Dexamethasone 46948-6
23565 Fludrocortisone 46949-4
23566 Flunisolide 46950-2
23567 Fluorometholone 46951-0
23568 Fluticasone Propionate 46952-8
23569 Megestrol Acetate 46953-6
23570 Methylprednisolone 46954-4
23571 Prednisolone 46955-1
23572 Prednisone 46956-9
23573 Triamcinolone 46957-7
23574 Triamcinolone Acetonide 46958-5

Clinical Information

Synthetic glucocorticoids are widely used and have important clinical utility both as anti-inflammatory and immunosuppressive agents. The medical use of these agents, as well as their surreptitious use, can sometimes lead to a confusing clinical presentation. Patients exposed to these steroids may present with clinical features of Cushing syndrome, but with suppressed cortisol levels and evidence of hypothalamus-pituitary-adrenal axis suppression.


The fluticasone propionate analyte is reported with this test and is also available separately, see 17BFP / Fluticasone 17-Beta-Carboxylic Acid, Urine for more information.


This test screens for and quantitates, if present, the following synthetic glucocorticoids: beclomethasone dipropionate, betamethasone, budesonide, dexamethasone, fludrocortisone, flunisolide, fluorometholone, megestrol acetate, methylprednisolone, prednisolone, prednisone, triamcinolone, and triamcinolone acetonide.


The presence of synthetic glucocorticoids in urine indicates current or recent use of these compounds. Since several of these compounds exceed the potency of endogenous cortisol by 1 or more orders of magnitude, even trace levels may be associated with Cushingoid features.

Analytic Time

3 days

Specimen Retention Time

3 months

Reject Due To









Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Stable Isotope Dilution Analysis