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HelpTest Code TBSP Mycobacterium tuberculosis Complex Species Identification, PCR, Varies
Infectious
Ordering Guidance
This test should be used to identify the species within the Mycobacterium tuberculosis complex from a known M tuberculosis complex isolate.
For identification of M tuberculosis complex from isolate growth, order CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies.
For rapid identification of M tuberculosis complex directly from a specimen, order MTBRP / Mycobacterium tuberculosis Complex, Molecular Detection, PCR, Varies or MTBXS / Mycobacterium tuberculosis Complex, Molecular Detection and Rifampin Resistance, PCR, Sputum.
Shipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and suspected organism identification are required.
Specimen Required
Specimen Type: Mycobacterium tuberculosis complex isolate growing in pure culture
Supplies: Infectious Container, Large (T146)
Container/Tube: Growth on solid slant media, eg, Middlebrook 7H10, 7H11 and Lowenstein Jensen; growth in broth medium, eg, Mycobacteria Growth Indicator Tube, 7H9 broth BACT/ALERT MP or VersaTREK
Specimen Volume: Isolate with visible growth on solid media; if broth is sent, 3 mL or more of broth culture
Collection Instructions:
1. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.
2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Additional Information: If subculture to Middlebrook agar medium is needed to ensure purity, turnaround time for results may be delayed.
Useful For
Determining the species of a Mycobacterium tuberculosis complex culture isolate
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RTBSP | Id, Mtb Speciation, PCR | No, (Bill Only) | Yes |
Testing Algorithm
When this test is ordered, species identification will always be performed at an additional charge.
Special Instructions
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
M tuberculosis species ID, PCRSpecimen Type
VariesSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Agar plate Mixed culture |
Reject |
Clinical Information
This assay provides a species-level identification of microbiologic culture isolates previously identified to be a member of the Mycobacterium tuberculosis complex. Species level identification can be important for patient care or for epidemiologic investigations. For example, the species-level identification of Mycobacterium bovis bacillus Calmette-Guerin (BCG) can assist with identification of disseminated infections following use of the vaccine as an adjuvant during chemotherapy.
Reference Values
Not applicable
Interpretation
This assay can differentiate the most common species within the Mycobacterium tuberculosis complex, which are, M tuberculosis, Mycobacterium bovis, Mycobacterium bovis bacillus Calmette-Guerin (BCG; the vaccine strain), Mycobacterium canettii, Mycobacterium caprae, Mycobacterium microti, and Mycobacterium pinnepedii. This assay cannot distinguish Mycobacterium africanum from Mycobacterium mungi so if that result is obtained, the organism will be reported as M africanum/M mungi.
Day(s) Performed
Monday through Friday
Report Available
7 to 14 daysSpecimen Retention Time
Subculture: 1 yearPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87150
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TBSP | M tuberculosis species ID, PCR | 94576-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TBSP | M tuberculosis species ID, PCR | 94576-6 |